Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Hampton Research HR2-723/1.0 M BICINE pH 9.0 - 100 ml
HR2-723/1.0 M BICINE pH 9.0 - 100 ml. Please note items from this supplier are non-returnable.
![Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 100 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661046.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 100 Tablets per Bottle
PBS [Phosphate Buffered Saline] 100 mL Tablets are solid tablets that dissolve easily to prepare 100 mL of phosphate-buffered saline. These tablets provide a convenient and efficient way to prepare PBS solutions, eliminating the need for weighing and measuring individual components. They are ideal for laboratory applications that require consistent and reliable buffer preparation, including cell culture, microbiological testing, and molecular biology procedures. The tablets dissolve quickly in water, ensuring that the resulting buffer maintains the correct pH and osmolarity for a wide range of applications. Each bottle contains 100 tablets, making it a cost-effective option for labs that require frequent PBS preparation.
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 120 Grams of Powder, Makes 25 Liters of Solution
Murashige & Skoog Medium with MES Buffer is a specialized medium formulated for plant tissue culture, supplied in a 25-liter format. With 120 grams of powder, this formulation makes 25 liters of nutrient solution. It is enhanced with MES buffer, which ensures that the pH remains stable throughout the culture process, providing optimal conditions for plant cell growth. This medium is ideal for large-scale plant tissue culture studies and experiments. The MES buffer stabilizes the pH, preventing fluctuations that can affect cellular processes, making it essential for consistent growth in laboratory and research environments. This medium is commonly used in biotechnology and plant regeneration projects.
Research Products International Corp TRIS, Buffered Saline, 500 ML
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
Research Products International Corp PBS, 1X Solution, 1.0ml Pre-Filled Tubes, S, 1000 CS
PBS (Phosphate-Buffered Saline) is a balanced salt solution that helps maintain the pH and osmolarity of experimental systems, making it ideal for various biological and biochemical experiments.
1X Solution refers to a diluted concentration of a particular solution. In the case of PBS, a 1X solution means that it is prepared by diluting the concentrated stock solution to a specific ratio, usually 1 part stock solution to 9 parts water or another suitable solvent. 1.0ml Pre-Filled Tubes are small tubes that come pre-filled with a specified volume of liquid, in this case, 1.0 milliliters of PBS. These tubes are convenient and sterile, ensuring the integrity and purity of the solution. They are commonly used in laboratory settings to provide a controlled and standardized amount of PBS for various applications.
![Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75070-25.0.JPG-250.jpg)
Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 25 Grams
HEPPSO (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) is a zwitterionic buffering agent commonly used particularly in biochemical and molecular biology research. It is valued for its ability to maintain a stable pH in a variety of biological assays and experiments.
HEPPSO is often preferred for its buffering capacity over a wide pH range, typically between pH 6.8 to 8.2, making it suitable for various applications such as protein purification, enzyme assays, cell culture, and electrophoresis.
Additionally, HEPPSO is less likely to interfere with biological processes compared to some other buffering agents, making it a versatile choice for maintaining optimal conditions in biological experiments.
Research Products International Corp TRIS BASE, 40% SOLUTION, 4 LITER
A 40% weight by volume solution of TRIS (3.3M TRIS Base) prepared in Molecular Grade Water.
![Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92060-25.0.JPG-250.jpg)
Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams
- Substance Name: 3-(N-Morpholino)2-hydroxypropanesulfonic acid
- CAS Number: 68399-77-9
- Molecular Formula: C7H15NO5S
- Molecular Weight: 225.3
Sigma Aldrich Fine Chemicals Biosciences MES hemisodium salt 98 tit100G
SODIUM MES hemisodium salt 98 tit100G
Quality Biological Inc Tris HCl (1 M), pH 7.5, 1000 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ These solutions of Tris Hydrochloride provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). ¿ Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ pH Range: 7.5 ¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Research Products International Corp PBS 1X SOLN 1ML TUBES 36/CS
Ready to use sterile 1X solution, supplied in sterile pre-filled screw cap tubes. Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. Each tube contains 1.0 ml PBS 1X Sterile Solution, packaged in a sealed white cardboard freezer box with a 6 x 6 divider. PBS is supplied in 4.5 ml screw cap tubes that are 3.5 “ high (cap on). Each case holds 36 pre-filled tubes.
Convenient 1.0 ml of PBS per tube
Sterile
36 x 1.0 ml
Hampton Research 100% TACSIMATE PH 8.0 200ML
HR2-829/100% Tacsimate pH 8.0 - 200 ml. Please note items from this supplier are non-returnable.